30 Dec 2008

1. The shotgun sequencing run of Lentinula edodes generates 115 Mbp data with an average of 250bp.

2. Emulsion PCR breaking and DNA beads enrichment were done for Pleurotus cDNA library (16 x 3 cpb and 16 x 3.5 cpb). The % good DNA beads recovery is a bit lower than expected and 6 additional emPCR is necessary.

Patrick